Citation Minion Virus 49+
African swine fever virus asfv is the causative agent of a severe and highly contagious viral disease of pigs that poses serious economic consequences to the swine industry due to the high mortality rate and impact on international trade. We performed metagenomic sequencing using both the illumina miseq and the portable oxford nanopore minion on clinical samples which were real time reverse transcription pcr qrt pcr positive for chikungunya chikv or dengue virus denv two of the most important arboviruses.
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Plos one 13 e0194366 Cite. There is no effective vaccine to control african swine fever asf and therefore efficient disease control is dependent on early detection and diagnosis. Early minion nanopore single molecule sequencing technology enables the characterization of hepatitis b virus genetic complexity in clinical samples. A total of 26 samples with a range of representative clinical ct. This technology was an improvement over sanger based sequencing of the single allele amplicons due to a booster amplification step in the.With the unique capabilities of third generation sequencing we investigated the application of oxford nanopore minion technology for rapid sequencing of the amplicons generated in the single allele assay. Sequencing libraries were generated by pcr free tagmentation in under 10 minutes and loaded onto a minion sequencer. 24 may Cite multiplex pcr method for minion and illumina sequencing of zika and other virus genomes directly from clinical samples. We designed an adapter to short highly conserved termini of the influenza a virus genome to target the sense rna into a protein nanopore on the oxford nanopore minion sequencing platform. Diagnosis and characterization of canine distemper virus through sequencing by minion nanopore technology.
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